Characterisation, Recombinant Expression and Immunogenicity of Bhlp29.7, an Outer Membrane Lipoprotein of Brachyspira Hyodysenteriae
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چکیده
منابع مشابه
The use of ELISAs for monitoring exposure of pig herds to Brachyspira hyodysenteriae
BACKGROUND Swine dysentery (SD), a mucohaemorrhagic diarrhoeal disease of pigs, results from infection of the large intestine with the spirochaete Brachyspira hyodysenteriae. ELISA systems using whole spirochaete cells (WC) and the B. hyodysenteriae outer membrane lipoprotein Bhlp29.7 previously have been established as potential diagnostic tools for SD. However, their true value in identifying...
متن کاملEvaluation of recombinant Bhlp29.7 as an ELISA antigen for detecting pig herds with swine dysentery.
Swine dysentery (SD) results from infection of the porcine large intestine with the anaerobic intestinal spirochaete Brachyspira hyodysenteriae. Diagnosis of SD traditionally has relied on detecting the spirochaete in the faeces of acutely affected pigs. To date simple and reliable serological assays that can be applied as a diagnostic tool at the herd level have not been available. In the curr...
متن کاملDifferential expression of the Bhmp39 major outer membrane proteins of Brachyspira hyodysenteriae.
The enteric, anaerobic spirochete Brachyspira hyodysenteriae is the causative agent of swine dysentery, a severe mucohemorrhagic diarrheal disease of pigs that has economic significance in every major pork-producing country. Recent investigation into potential vaccine candidates has focused on the outer membrane proteins of B. hyodysenteriae. Bhmp39 (formerly Vsp39) is the most abundant surface...
متن کاملDevelopment of a serological ELISA using a recombinant protein to identify pig herds infected with Brachyspira hyodysenteriae.
Brachyspira hyodysenteriae is an anaerobic spirochaete that can induce swine dysentery (SD), a severe mucohaemorrhagic colitis in grower and fattener pigs. The aim of this study was to develop a serological ELISA for use as a screening method to detect evidence of herd infection. Bioinformatic analysis of the complete genome sequence of strain WA1 was used to identify genes predicted to encode ...
متن کاملSerologic detection of Brachyspira (Serpulina) hyodysenteriae infections.
Swine dysentery (SD) caused by the intestinal spirochete Brachyspira hyodysenteriae is an economically important disease in pig-producing countries throughout the world. To date, no specific serologic assay is commercially available for the diagnosis of pigs with SD. Several serologic techniques have been identified in the past; however, these tests have all used either whole-cell proteins or l...
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